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Selected Publications

2022

USP1-trapping lesions as a source of DNA replication stress and genomic instability

Coleman KE, Yin Y, Kit Leng Lui S, Keegan S, Fenyo D, Smith DJ, Rothenberg E, Huang TT

Nature Communications (2022)

Single-cell transcriptomics identifies Gadd45b as a regulator of herpesvirus-reactivating neurons

Hu H-L, Srinivas KP, Shuoshuo W, Chao MV, Lionnet T, Mohr I, Wilson AC, Depledge DP, Huang TT

EMBO Reports (2022)

2021

A molecular sensor determines the ubiquitin substrate specificity of SARS-CoV-2 papain-like protease

Patchett S, Lv Z, Rut W, Bekes M, Drag M, Olsen SK, Huang TT

Cell Reports (2021)

Single-molecule imaging reveals replication fork coupled formation of G-quadruplex structures hinders local replication stress signaling

Lee WTC, Yin Y, Morten MJ, Tonzi P, Gwo PP, Odermatt DC, Modesti M, Cantor SB, Gari K, Huang TT, Rothenberg E

Molecular Cell (2021)

A basal-level activity of ATR links replication fork surveillance and stress response

Yin Y, Lee WTC, Gupta D, Tonzi P, Borowiec JA, Huang TT, Modesti M, Rothenberg E

Molecular Cell (2021)

WEE1i-ATRi combination therapy: a promising low-dose treatment for CCNE1-amplified gynecologic cancers

Goehring L, Huang TT

Cell Reports Medicine Preview (2021)

Monitoring genome-wide replication fork directionality by Okazaki fragment sequencing in mammalian cells

Kit Leng Lui S, Keegan S, Tonzi P, Kahli M, Chen Y-H, Chalhoub N, Coleman KE, Fenyo D, Smith D, Huang TT

Nature Protocols (2021)

2020

Hyperactive CDK2 activity in basal-like breast cancer imposes a genome integrity liability that can be exploited by targeting DNA polymerase E

Sviderskiy V, Blumenberg L, Gorodetsky E, Karakousi TR, Hirsh N, Alvarez SW, Terzi EM, Kaparos E, Whiten GC, Ssebyala S, Tonzi P, Mir H, Neel BG, Huang TT, Adams S, Ruggles KV, Possemato R

Molecular Cell (2020)

Roles for MDC1 in cancer development and treatment

Ruff S, Logan SK, Garabedian MJ, Huang TT

DNA Repair (2020)

Transcription-replication conflicts as a source of common fragile site instability caused by BMI1-RNF2 deficiency

Sanchez A, de Vivo A, Tonzi P, Kim J, Huang TT, Kee Y

PLoS Genetics (2020)

Activity profiling and crystal structures of inhibitor-bound SARS-CoV-2 papain-like protease: A framework for anti-COVID-19 drug design

Rut W, Lv Z, Zmudzinski M, Patchett S, Nayak D, Snipas S, El Oualid F, Huang TT, Bekes M, Drag M, Olsen SK

Science Advances (2020)

Using Primary SCG Neuron Cultures to Study Molecular Determinants of HSV-1 Latency and Reactivation

Hu H-L, Srinivas KP, Mohr I, Huang TT, Wilson AC

Herpes Simplex Virus (2020)

2019

Role of Y-family translesion DNA polymerases in replication stress: Implications for new cancer therapeutic targets

Tonzi P, Huang TT

DNA Repair (2019)

TOP2b-dependent nuclear DNA damage shapes extracellular growth factor responses via dynamic AKT phosphorylation to control virus latency

Hu H-L, Shiflett LA, Kobayashi M, Chao MV, Wilson AC, Mohr I, Huang TT

Molecular Cell (2019)  

Transcription shapes DNA replication initiation and termination in human cells​

Chen YH, Keegan S, Kahli M, Tonzi P, Fenyo D, Huang TT, Smith, DJ

Nature Structural & Molecular Biology (2019) 

2018

Translesion polymerase kappa-dependent DNA synthesis underlies replication fork recovery

Tonzi P, Yin Y, Lee CWT, Rothenberg E, Huang, TT

Elife (2018)

In a Class of Its Own: A New Family of Deubiquitinases Promotes Genome Stability

Coleman KE; Huang TT

Molecular Cell (2018)

The COP9 signalosome inhibits Cullin-RING E3 ubiquitin ligases independently of its deneddylase activity

Suisse A, Bekes M, Huang TT; Treisman JE

Fly (2018)

2017

SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function

Coleman KE, Bekes M, Chapman JR, Crist SB, Jones MJK, Ueberheide BM, Huang TT

ELife (2017)

2016

HUWE1 comes to the rescue at stalled replication forks

Coleman KE, Huang TT

EMBO Reports (2016)

Recognition of Lys48-Linked Di-ubiquitin and Deubiquitinating Activities of the SARS Coronavirus Papain-like Protease

Bekes M, van der Heden vN, Gerbrand J, Ekkebus R, Ovaa H, Huang TT, Lima CD

Molecular Cell (2016)

How SUMOylation Fine-Tunes the Fanconi Anemia DNA Repair Pathway

Coleman KE, Huang TT

Frontiers In Genetics (2016)

Role of Deubiquitinating Enzymes in DNA Repair

Kee Y, Huang TT

Molecular & Cellular Biology (2016)

2015

ATR-mediated phosphorylation of FANCI regulates dormant origin firing in response to replication stress

Chen YH, Jones MJK, Yin Y, Crist SB, Colnaghi L, Sims RJ, Rothenberg E, Jallepalli PV, Huang TT

Molecular Cell (2015) 

SARS hCoV papain-like protease is a unique Lys48 linkage-specific di-distributive deubiquitinating enzyme

Bekes M, Rut W, Kasperkiewicz P, Mulder MP, Ovaa H, Drag M, Lima CD, Huang TT

Biochem J. (2015)

2014

Co-opting the Fanconi Anemia Genomic Stability Pathway Enables Herpesvirus DNA Synthesis and Productive Growth

Karttunen H, Savas JN, McKinney C, Chen YH, Yates JR, Hukkanen V, Huang TT, Mohr I

Molecular Cell (2014)

2013

DUB-Resistant Ubiquitin to Survey Ubiquitination Switches in Mammalian Cells

Bekes M, Okamoto K, Crist SB, Jones MJ, Chapman JR, Brasher BB, Melandri FD, Ueberheide BM, Lazzerini Denchi E, Huang TT

Cell Reports (2013)

2012

Deubiquitinases as a Signaling Target of Oxidative Stress

Cotto-Rios XM, Bekes M, Chapman J, Ueberheide B, Huang TT

Cell Reports (2012)

The Auto-Generated Fragment of the Usp1 Deubiquitylase Is a Physiological Substrate of the N-End Rule Pathway

Piatkov KI, Colnaghi L, Bekes M, Varshavsky A, Huang TT

Molecular Cell (2012)

The Fanconi anemia pathway in replication stress and DNA crosslink repair

Jones MJK, Huang TT

Cellular & Molecular Life Sciences: CMLS (2012)

Dysregulation of DNA polymerase κ recruitment to replication forks results in genomic instability

Jones MJ, Colnaghi L, Huang TT

EMBO J. (2012)

2011

Insights into phosphorylation-dependent mechanisms regulating USP1 protein stability during the cell cycle

Cotto Rios XM, Jones MJ, Huang TT

Cell Cycle (2011)

APC/CCdh1-dependent proteolysis of USP1 regulates the response to UV-mediated DNA damage

Cotto-Rios XM; Jones MJK, Busino L, Pagano M, Huang TT

Journal of Cell Biology (2011)

Patient-derived C-terminal mutation of FANCI causes protein mislocalization and reveals putative EDGE motif function in DNA repair

Colnaghi L, Jones MJK, Cotto-Rios XM, Schindler D, Hanenberg H, Huang TT

Blood (2011)

2007

FANCI is a second monoubiquitinated member of the Fanconi anemia pathway

Sims, AE, Spiteri E, Sims RJ, Arita AG, Lach FP, Landers T, Wurm M, Freund M, Neveling K, Hanenberg H, Auerbach AD, Huang, Tony T

Nature Structural & Molecular Biology (2007)

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